rabbit polyclonal anti occludin (Proteintech)
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Proteintech
rabbit polyclonal anti occludin
Rabbit Polyclonal Anti Occludin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+occludin/Occludin+Antibody/pm41657037-57-25-32
Average 96 stars, based on 1555 article reviews
Rabbit Polyclonal Anti Occludin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+occludin/Occludin+Antibody/pm41657037-57-25-32
Average 96 stars, based on 1555 article reviews
rabbit polyclonal anti occludin - by Bioz Stars,
2026-09
96/100 stars
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Incubation:Article Title: A novel histone deacetylase inhibitor W2A-16 improves the barrier integrity in brain vascular endothelial cells Article Snippet: After fixation, cells were permeabilized with PBS containing 0.3% Triton X-100 (Sigma-Aldrich, Cat No. X100) and blocked with Protein Block Serum-Free Ready-To-Use (Agilent Technologies, Cat No. X0909, Santa Clara, CA, United States). .. Cells were incubated with primary antibodies in background-reducing dilution buffer (Agilent Technologies, Cat No. S3022) overnight at 4°C with the following primary antibodies: mouse monoclonal anti-ZO-1 (1: 200 dilution; Invitrogen, Cat No. 33–9,100, Carlsbad, CA, United States, clone ZO1-1A12), mouse monoclonal anti-claudin-5 (1:200 dilution; Invitrogen, Cat No. 35–2,500, clone 4C3C2), mouse monoclonal anti-CD31 (1:50 dilution; R&D Systems, Cat No. BBA7, Minneapolis, USA, clone 9G11), Article Title: Foodborne PET Microplastic Contamination Compromises Intestinal Barrier through a Mitochondrial-AMPK-DNA Damage Pathway. Article Snippet: Poly(ethylene terephthalate) microplastics (PET-MPs), prevalent dietary contaminants, pose potential risks to intestinal health; yet underlying mechanisms of sustained exposure remain poorly defined.. We evaluated the toxicity of gastrointestinal-digested PET-MPs in a human intestinal epithelial coculture model for 24 h. Digested PET-MPs triggered cytotoxicity, oxidative stress, barrier disruption, and dysregulated cytokines, impairing epithelial homeostasis.. Untargeted metabolomics identified the AMPK signaling pathway as a perturbed node. Article Title: Prevotella copri transplantation promotes neurorehabilitation in a mouse model of traumatic brain injury Article Snippet: The sample proteins (20 μg/lane) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen) and transferred onto polyvinylidene uoride (PVDF) membranes (Millipore, Boston, MA, USA). .. Membranes were blocked with 5% nonfat milk for 1 hour at room temperature and then incubated overnight at 4°C with primary antibodies, including rabbit monoclonal anti-β-actin (1:5000, ab213262, Abcam), rabbit polyclonal antiZO-1 (1:1000, 21773-1-AP, Proteintech), Article Title: Prevotella copri transplantation promotes neurorehabilitation in a mouse model of traumatic brain injury Article Snippet: The sample proteins (20 μg/lane) were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS‒PAGE) (Invitrogen) and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Boston, MA, USA). .. Membranes were blocked with 5% nonfat milk for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies, including rabbit monoclonal anti-β-actin (1:5000, ab213262, Abcam), rabbit polyclonal anti-ZO-1 (1:1000, 21773-1-AP, Proteintech), Article Title: Live and pasteurized Akkermansia muciniphila decrease susceptibility to Salmonella Typhimurium infection in mice. Article Snippet: Then the sample was transferred to nitrocellulose membranes (Life Technologies, USA) in ice at a constant current 300 mA for 90 min. .. The nitrocellulose membranes were incubated with primary antibodies overnight at 4 C. The information of primary antibodies was listed as follows: rabbit polyclonal antiMucin 2 (1:1000; Proteintech, China), rabbit polyclonal anti-ZO1 (1:1000; Abclonal, China), Blocking Assay:Article Title: Foodborne PET Microplastic Contamination Compromises Intestinal Barrier through a Mitochondrial-AMPK-DNA Damage Pathway. Article Snippet: Poly(ethylene terephthalate) microplastics (PET-MPs), prevalent dietary contaminants, pose potential risks to intestinal health; yet underlying mechanisms of sustained exposure remain poorly defined.. We evaluated the toxicity of gastrointestinal-digested PET-MPs in a human intestinal epithelial coculture model for 24 h. Digested PET-MPs triggered cytotoxicity, oxidative stress, barrier disruption, and dysregulated cytokines, impairing epithelial homeostasis.. Untargeted metabolomics identified the AMPK signaling pathway as a perturbed node. Control:Article Title: Foodborne PET Microplastic Contamination Compromises Intestinal Barrier through a Mitochondrial-AMPK-DNA Damage Pathway. Article Snippet: Poly(ethylene terephthalate) microplastics (PET-MPs), prevalent dietary contaminants, pose potential risks to intestinal health; yet underlying mechanisms of sustained exposure remain poorly defined.. We evaluated the toxicity of gastrointestinal-digested PET-MPs in a human intestinal epithelial coculture model for 24 h. Digested PET-MPs triggered cytotoxicity, oxidative stress, barrier disruption, and dysregulated cytokines, impairing epithelial homeostasis.. Untargeted metabolomics identified the AMPK signaling pathway as a perturbed node. |
![Acetate reverses chitosan-mediated repair of the intestinal barrier, increased inflammation in the colon, plasma, and SN, and promotes microglia activation in an MPTP-induced mouse model of PD. (A) Colon length ( n = 5/group). (B) ZO-1 and occludin expression, as assessed by western blot ( n = 3/group). All target proteins were normalized to the reference protein GAPDH. Compared with the chitosan group, acetate supplementation reduced ZO-1 and occludin expression levels. (C) Immunofluorescence staining for ZO-1 (green, Alexa Fluor 488) and occludin (red, Alexa Fluor 594) in mouse colon tissue ( n = 3/group). The immunofluorescence results were consistent with the western blot results. Scale bars: 10 μm. (D) The relative mRNA levels of IL-1β, IL-6, IL-8, IL-10, TNF-α, and iNOS in mouse colon tissue were measured by QPCR ( n = 3/group). Compared with the chitosan group, acetate supplementation resulted in an increase in IL-1β, IL-6, IL-10, TNF-α, and iNOS expression levels in the colon. The data shown in B-D were normalized to the control group. (E) The expression levels of inflammatory cytokines, including IL-1β, IL-6, IL-10, and TNF-α, in mouse plasma were measured by ELISA ( n = 5/group). Compared with the chitosan group, IL-1β and TNF-α levels were significantly increased in the plasma of the acetate group, while IL-10 expression was significantly decreased. (F) The mRNA levels of IL-1β, IL-6, IL-8, IL-10, TNF-α, and iNOS (normalized to the control group) in mouse SN tissue were determined via QPCR ( n = 3/group). Treatment with acetate enhanced TNF-α expression and decreased IL-6 and IL-10 expression in the SN. (G) Representative images of immunofluorescence staining for Iba1 (green, Alexa Fluor 488) and TH (red, Alexa Fluor 594) in the SN ( n = 3/group). The chitosan group exhibited fewer microglia than the MPTP group, while the chitosan + acetate group exhibited more microglia than the chitosan-only group. Scale bars: 50 μm. All data are presented as the mean ± SD. All experiments were repeated at least three times. * P < 0.05 (one-way analysis of variance followed by Tukey’s multiple comparisons test [A–C, G] or unpaired t -test [D–F]). DAPI: 4′,6-Diamidino-2-phenylindole; ELISA: enzyme-linked immunosorbent assay; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Iba1: ionized calcium-binding adapter molecule 1; IL-1β: interleukin-1 beta; IL-6: interleukin-6; IL-8: interleukin-8; IL-10: interleukin-10; iNOS: inductible nitric oxide synthase; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; n.s.: no significance; NaA: sodium acetate; PD: Parkinson’s disease; QPCR: quantitative polymerase chain reaction; SN: substantia nigra; TH: tyrosine hydroxylase; TNF-α: tumor necrosis factor alpha; ZO-1: Zonula occludens-1.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4542/pmc12094542/pmc12094542__NRR-21-377-g006.jpg)
